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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
07/12/2016 |
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Data da última atualização: |
31/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, A. R.; SILVA, G. F.; GUALBERTO, G. F.; ALMEIDA, F. L. |
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Afiliação: |
A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA. |
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Título: |
Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016. |
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DOI: |
http://dx.doi.org/10.4238/gmr15049228 |
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Idioma: |
Inglês |
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Conteúdo: |
Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR. |
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Thesagro: |
Colossoma Macropomum; Tambaqui. |
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Thesaurus Nal: |
aquaculture; molecular biology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153400/1/gmr-15-04-gmr.15049228.pdf
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Marc: |
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024 7 $ahttp://dx.doi.org/10.4238/gmr15049228$2DOI
100 1 $aNASCIMENTO, A. R.
245 $aValidation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).$h[electronic resource]
260 $c2016
520 $aTambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
650 $aaquaculture
650 $amolecular biology
650 $aColossoma Macropomum
650 $aTambaqui
700 1 $aSILVA, G. F.
700 1 $aGUALBERTO, G. F.
700 1 $aALMEIDA, F. L.
773 $tGenetics and Molecular Research$gv. 15, n. 4, p. 15049228, 2016.
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Embrapa Amazônia Ocidental (CPAA) |
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